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guide ittm mutation detection kit  (TaKaRa)


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    Structured Review

    TaKaRa guide ittm mutation detection kit
    Guide Ittm Mutation Detection Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutation+detection+kit/Guide-it+Mutation+Detection+Kit/10__1016_slash_j__jmgm__2026__109445-120-6-10
    Average 95 stars, based on 138 article reviews
    guide ittm mutation detection kit - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: MIR408-encoded peptide, miPEP408, regulates cadmium stress response through sulfur assimilation pathway.
    Article Snippet: • MIR408 encodes small peptide, miPEP408, which regulates miR408

    Amplification:

    Article Title: MIR408-encoded peptide, miPEP408, regulates cadmium stress response through sulfur assimilation pathway.
    Article Snippet: • MIR408 encodes small peptide, miPEP408, which regulates miR408

    Mutagenesis:

    Article Title: MIR408-encoded peptide, miPEP408, regulates cadmium stress response through sulfur assimilation pathway.
    Article Snippet: • MIR408 encodes small peptide, miPEP408, which regulates miR408

    Article Title: Loss of Calpain-2 attenuates TGF-β-driven Epithelial-Mesenchymal transition and invasive behavior in A549 lung cancer cells.
    Article Snippet: Lung cancer accounts for the highest number of cancer-related deaths globally, largely due to its high propensity for early and widespread metastasis.. Calpains, a family of calcium-dependent cysteine proteases, are involved in various physiological processes, including cytoskeletal remodeling, migration, and invasion.. Among them, calpain-2 has been implicated in promoting cancer cell invasiveness and metastatic behavior, although its precise role in non-small cell lung cancer (NSCLC) remains poorly understood.

    Article Title: PD-L1 regulates c-MET phosphorylation and contributes to MET-dependent resistance to osimertinib in EGFR-mutant NSCLC
    Article Snippet: sgRNAs were designed using the Guide-Design-Resources tool ( https://zlab.bio/guide-design-resources ), and cloned into CRISPR plasmid (pSpCas9(BB)-2A-Puro, Addgene) by BbsI sites. .. Plasmids were transfected into HEK293T cells, and the sgRNA activity was assessed using Guide-it Mutation Detection Kit (Takara Bio) according to the manufacturer’s instruction. ..

    Article Title: PD-L1 regulates c-MET phosphorylation and contributes to MET-dependent resistance to osimertinib in EGFR-mutant NSCLC.
    Article Snippet: Establishment of PD‐L1 knock‐out (KO) lines using CRISPR system sgRNAs were designed using the Guide-DesignResources tool (https:// zlab. bio/ guide- design- resou rces), and cloned into CRISPR plasmid (pSpCas9(BB)-2A-Puro, Addgene) by BbsI sites. .. Plasmids were transfected into HEK293T cells, and the sgRNA activity was assessed using Guide-it Mutation Detection Kit (Takara Bio) according to the manufacturer’s instruction. ..

    Article Title: Delayed inactivation of TRPC6 as a determinative characteristic of FSGS-associated variants
    Article Snippet: A bright green fluorescent protein, ZsGreen1, expressed clones were isolated by BD FACS Melody cell sorter (BD Biosciences). .. After single-cell isolation, genomic DNA was extracted using the Guide-it Mutation Detection Kit (Clontech). ..

    Article Title: Transmembrane Serine Protease TMPRSS11B promotes an acidified tumor microenvironment and immune suppression in lung squamous cell carcinoma
    Article Snippet: .. The assay was performed on Tmprss11b knockout and non-targeting control KLN205 cells using Guide-it TM Mutation Detection kit (Takara, Cat No. 631448) following manufacturer’s protocol. ..

    Article Title: Delayed inactivation of TRPC6 as a determinative characteristic of FSGS-associated variants.
    Article Snippet: A bright green fluorescent protein, ZsGreen1 expressed clones were isolated by BD FACS Melody cell sorter (BD Biosciences). .. After single-cell isolation, genomic DNA was extracted using the Guide-it Mutation Detection Kit (Clontech). ..

    CRISPR:

    Article Title: Loss of Calpain-2 attenuates TGF-β-driven Epithelial-Mesenchymal transition and invasive behavior in A549 lung cancer cells.
    Article Snippet: Lung cancer accounts for the highest number of cancer-related deaths globally, largely due to its high propensity for early and widespread metastasis.. Calpains, a family of calcium-dependent cysteine proteases, are involved in various physiological processes, including cytoskeletal remodeling, migration, and invasion.. Among them, calpain-2 has been implicated in promoting cancer cell invasiveness and metastatic behavior, although its precise role in non-small cell lung cancer (NSCLC) remains poorly understood.

    other:

    Article Title: TMPRSS11B promotes an acidified microenvironment and immune suppression in squamous lung cancer
    Article Snippet: Magnetic resonance imaging (MRI) was performed on mice using the 7 T Bruker Biospec instrument at the Advanced Imaging Research Center at UT Southwestern with the help of Dr. Janaka Wansapura.

    Transfection:

    Article Title: PD-L1 regulates c-MET phosphorylation and contributes to MET-dependent resistance to osimertinib in EGFR-mutant NSCLC
    Article Snippet: sgRNAs were designed using the Guide-Design-Resources tool ( https://zlab.bio/guide-design-resources ), and cloned into CRISPR plasmid (pSpCas9(BB)-2A-Puro, Addgene) by BbsI sites. .. Plasmids were transfected into HEK293T cells, and the sgRNA activity was assessed using Guide-it Mutation Detection Kit (Takara Bio) according to the manufacturer’s instruction. ..

    Article Title: PD-L1 regulates c-MET phosphorylation and contributes to MET-dependent resistance to osimertinib in EGFR-mutant NSCLC.
    Article Snippet: Establishment of PD‐L1 knock‐out (KO) lines using CRISPR system sgRNAs were designed using the Guide-DesignResources tool (https:// zlab. bio/ guide- design- resou rces), and cloned into CRISPR plasmid (pSpCas9(BB)-2A-Puro, Addgene) by BbsI sites. .. Plasmids were transfected into HEK293T cells, and the sgRNA activity was assessed using Guide-it Mutation Detection Kit (Takara Bio) according to the manufacturer’s instruction. ..

    Activity Assay:

    Article Title: PD-L1 regulates c-MET phosphorylation and contributes to MET-dependent resistance to osimertinib in EGFR-mutant NSCLC
    Article Snippet: sgRNAs were designed using the Guide-Design-Resources tool ( https://zlab.bio/guide-design-resources ), and cloned into CRISPR plasmid (pSpCas9(BB)-2A-Puro, Addgene) by BbsI sites. .. Plasmids were transfected into HEK293T cells, and the sgRNA activity was assessed using Guide-it Mutation Detection Kit (Takara Bio) according to the manufacturer’s instruction. ..

    Article Title: PD-L1 regulates c-MET phosphorylation and contributes to MET-dependent resistance to osimertinib in EGFR-mutant NSCLC.
    Article Snippet: Establishment of PD‐L1 knock‐out (KO) lines using CRISPR system sgRNAs were designed using the Guide-DesignResources tool (https:// zlab. bio/ guide- design- resou rces), and cloned into CRISPR plasmid (pSpCas9(BB)-2A-Puro, Addgene) by BbsI sites. .. Plasmids were transfected into HEK293T cells, and the sgRNA activity was assessed using Guide-it Mutation Detection Kit (Takara Bio) according to the manufacturer’s instruction. ..

    Single Cell:

    Article Title: Delayed inactivation of TRPC6 as a determinative characteristic of FSGS-associated variants
    Article Snippet: A bright green fluorescent protein, ZsGreen1, expressed clones were isolated by BD FACS Melody cell sorter (BD Biosciences). .. After single-cell isolation, genomic DNA was extracted using the Guide-it Mutation Detection Kit (Clontech). ..

    Article Title: Delayed inactivation of TRPC6 as a determinative characteristic of FSGS-associated variants.
    Article Snippet: A bright green fluorescent protein, ZsGreen1 expressed clones were isolated by BD FACS Melody cell sorter (BD Biosciences). .. After single-cell isolation, genomic DNA was extracted using the Guide-it Mutation Detection Kit (Clontech). ..

    Isolation:

    Article Title: Delayed inactivation of TRPC6 as a determinative characteristic of FSGS-associated variants
    Article Snippet: A bright green fluorescent protein, ZsGreen1, expressed clones were isolated by BD FACS Melody cell sorter (BD Biosciences). .. After single-cell isolation, genomic DNA was extracted using the Guide-it Mutation Detection Kit (Clontech). ..

    Article Title: Delayed inactivation of TRPC6 as a determinative characteristic of FSGS-associated variants.
    Article Snippet: A bright green fluorescent protein, ZsGreen1 expressed clones were isolated by BD FACS Melody cell sorter (BD Biosciences). .. After single-cell isolation, genomic DNA was extracted using the Guide-it Mutation Detection Kit (Clontech). ..

    Knock-Out:

    Article Title: Transmembrane Serine Protease TMPRSS11B promotes an acidified tumor microenvironment and immune suppression in lung squamous cell carcinoma
    Article Snippet: .. The assay was performed on Tmprss11b knockout and non-targeting control KLN205 cells using Guide-it TM Mutation Detection kit (Takara, Cat No. 631448) following manufacturer’s protocol. ..

    Control:

    Article Title: Transmembrane Serine Protease TMPRSS11B promotes an acidified tumor microenvironment and immune suppression in lung squamous cell carcinoma
    Article Snippet: .. The assay was performed on Tmprss11b knockout and non-targeting control KLN205 cells using Guide-it TM Mutation Detection kit (Takara, Cat No. 631448) following manufacturer’s protocol. ..



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